Nitrous Oxide (N2O)

Background

  • Commonly known as laughing gas, ‘nangs’ or ‘whippits.’ It belongs to the inhalant class of drugs.  

  • Can be purchased by anyone 18 or older, typically labeled ‘for food purposes only.’ 

  • Legally used in clinical settings like dentist, fuel booster in car racing, and whipped cream propellant 

  • Increasingly popular as recreational substance in teenagers and young adults. 

  • In the USA, 13 million people report using it in their lifetime.  

  • Marked increase in Mortality Rate in the US from 2010-2023: 0.01/100,000 population to 0.06/100,000 population (600% increase)  

 

Pharmacology/Pharmacokinetics 

  • Inhalational anesthetic, analgesic and anxiolytic, rapid onset and disappearing within minutes 

  • Interacts with opioid system, and also is an NMDA antagonist which can cause dopamine disinhibition  

  • This is the main target for anesthetic effect 

  • Abused for its euphoric and hallucinogenic effect lasting 5-20 seconds.  

  • Chronic use associated with neurologic sequalae including neuronal demyelination, Vitamin B12 deficiency causing subacute combined spinal cord degeneration 

  • Inactivates co-factor cobalamin and ultimately leads to disrupted DNA/RNA methylation needed for myelin sheath, blockade of NMDA receptors, and accumulation of reactive oxygen species, in addition to a cascade of metabolic effects that contribute to neurotoxicity.  

  • Gait instability, limb or generalized weakness, paresthesias are initial symptoms  

  • Psychiatric manifestations: hallucinations, anxiety, depression, delirium, memory impairment 

  • Hematologic sequalae are also possible: megaloblastic/pernicious anemias, myelosuppression 

  • Also associated with pulmonary embolism, central venous sinus thrombosis, and DVT 

 

Medical Management 

  • Usual ED supportive care for acute overdose although unlikely given short duration of effects: ABC’s, hemodynamic support 

  • Lesions can be seen on spinal cord MRI  

  • Indirect biomarkers can provide clues that individual may be abusing: Vitamin B12, plasma homocysteine, plasma MMA 

  • For chronic use, treatment involves permanent cessation of nitrous use and high dose IM vitamin B12; prognosis for full recovery of neuropathies is poor 

  • Naltrexone may be helpful in cases of chronic abuse 

Withdrawal 

  • No specific withdrawal syndrome reported 

 

References 

1. Allan J, Cameron J, Bruno J. A Systematic Review of Recreational Nitrous Oxide Use: Implications for Policy, Service Delivery and Individuals. International Journal of Environmental Research and Public Health. 2022;19(18):11567. doi:10.3390/ijerph191811567 

2.Brunt TM, van den Brink W, van Amsterdam J. Mechanisms Involved in the Neurotoxicity and Abuse Liability of Nitrous Oxide: A Narrative Review. Int J Mol Sci. 2022;23(23):14747. doi:10.3390/ijms232314747 

3. Garakani A, Jaffe RJ, Savla D, et al. Neurologic, psychiatric, and other medical manifestations of nitrous oxide abuse: A systematic review of the case literature. The American Journal on Addictions. 2016;25(5):358-369. doi:10.1111/ajad.12372 

4. Gernez E, Lee GR, Niguet JP, Zerimech F, Bennis A, Grzych G. Nitrous Oxide Abuse: Clinical Outcomes, Pharmacology, Pharmacokinetics, Toxicity and Impact on Metabolism. Toxics. 2023;11(12):962. doi:10.3390/toxics11120962 

5. Vohra V. Notes from the Field: Recreational Nitrous Oxide Misuse — Michigan, 2019–2023. MMWR Morb Mortal Wkly Rep. 2025;74. doi:10.15585/mmwr.mm7412a3 

6. Yockey RA, Hoopsick RA. US Nitrous Oxide Mortality. JAMA Netw Open. 2025;8(7):e2522164. doi:10.1001/jamanetworkopen.2025.22164 

7. Zaloum SA, Mair D, Paris A, et al. Tackling the growing burden of nitrous oxide-induced public health harms. The Lancet Public Health. 2025;10(3):e257-e263. doi:10.1016/S2468-2667(24)00298-6 

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